HiMedia – M2073 HiCrome™ EC Broth w/ RUG

Principle and Interpretation
Escherichia coli is a member of faecal coliform group of bacteria. It is a member of the indigenous faecal flora of warmblooded animals. E.coli is considered a specific indicator of faecal contamination and the possible presence of enteric pathogens. E.coli can be reliably detected with media that contain a chromogenic or fluorogenic substrate for beta glucuronidase, an enzyme that occurs almost exclusively in E. coli . s Resorufin-beta-D-glucuronic acid methyl ester (RUG) is a highly sensitive chromogenic and fluorogenic indicator for E.coli. In contrast to MUG, RUG is more specific and does not require fluorescent detection. The released dye Resorufin itself gives intense pink color which can be visually detected. Additional confirmation can be done by observation of fluorescence under uv light . Yeast extract and Acicase provides carbonaceous, nitrogenous substances, long chain amino acids, vitamins and other essential nutrients. Sodium chloride maintains osmotic equilibrium. The medium has a strong buffering system to control the pH in the medium. Grampositive bacteria especially Staphylococcus, Bacillus species and faecal Streptococcus are inhibited.

原理與解釋
大腸桿菌是糞便大腸菌群的一員。它是溫血動物的土著糞便菌群的成員。大腸桿菌被認為是糞便污染和腸道病原體可能存在的特定指標。大腸桿菌可以用含有β-葡醣醛酸酶的生色或熒光底物的培養基可靠地檢測，β-葡萄醣醛酸酶幾乎只在大腸桿菌中發生。 s Resorufin-beta-D-glucuronic acid methyl ester（RUG）是一種高度敏感的大腸桿菌髮色和熒光指示劑。與MUG相比，RUG更具特異性，不需要熒光檢測。 釋放的染料Resorufin本身產生強烈的粉紅色，可以在視覺上檢測到。可以通過在紫外光下觀察熒光來進行額外的確認。 酵母提取物和Acicase提供碳質，含氮物質，長鏈氨基酸，維生素和其他必需營養素。氯化鈉保持滲透平衡。該培養基具有強大的緩衝系統以控制培養基中的pH。革蘭氏陽性菌尤其是葡萄球菌，芽孢桿菌和糞鏈球菌受到抑制。