HIMEDIA HiCrome 顯色培養基（／產色培養基／呈色培養基） |
顯色培養基（／產色培養基／呈色培養基）
( Chromogenic / Fluorogenic Culture Media )
顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
(一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形，
藉此直接觀察菌落顏色即可對菌種做出鑑定，
其反應的靈敏度和特異性大大優於傳統培養基。
顯色培養基通常為乾粉狀，容易儲存。
加蒸餾水溶解後，部分產品無須高壓滅菌，培養時間依具體培養基而定，
通常是18-24小時，比傳統時間顯著縮短。
HiMedia – M2065 HiCrome™ Lactobacillus Selective Agar Base |
Recommended for isolation and differentiation between various species of Lactobacillus from a mixed culture by chromogenic method.
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Principle and Interpretation
Lactobacillus is a genus of Gram-positive, facultative anaerobic or microaerophilic, rod-shaped, non-spore-forming bacteria. They are a major part of the lactic acid bacteria group. As more LABs have been developed and sold in mixed forms as probiotics, it is necessary to develop a method for counting each LAB in a mixture(1) The medium contains peptone and HM extract, which provide nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other essential nutrients. Mannitol serves as the fermentable carbohydrate, fermentation of which can be detected by phenol red. M-protein aids in detecting casein hydrolysis activity. The chromogenic mixture present in the medium is cleaved by the enzyme b-glucosidase resulting in greenish blue to blue coloured colonies. For selective isolation of Lactobacillus, Ciprofloxacin supplement is added (FD345) which inhibits the accompanying bacteria.
Lactobacillus is a genus of Gram-positive, facultative anaerobic or microaerophilic, rod-shaped, non-spore-forming bacteria. They are a major part of the lactic acid bacteria group. As more LABs have been developed and sold in mixed forms as probiotics, it is necessary to develop a method for counting each LAB in a mixture(1) The medium contains peptone and HM extract, which provide nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other essential nutrients. Mannitol serves as the fermentable carbohydrate, fermentation of which can be detected by phenol red. M-protein aids in detecting casein hydrolysis activity. The chromogenic mixture present in the medium is cleaved by the enzyme b-glucosidase resulting in greenish blue to blue coloured colonies. For selective isolation of Lactobacillus, Ciprofloxacin supplement is added (FD345) which inhibits the accompanying bacteria.
原理與解釋
乳桿菌屬於革蘭氏陽性，兼性厭氧或微需氧桿狀桿狀非孢子形成細菌。 它們是乳酸菌群的主要部分。 隨著越來越多的LAB以混合形式開發和銷售為益生菌，有必要開發一種計算混合物中每種LAB的方法（1） 該培養基含有蛋白腖和HM提取物，提供含氮和含碳化合物，長鏈氨基酸，維生素和其他必需營養素。 甘露醇用作可發酵的碳水化合物，其發酵可以通過酚紅檢測。 M蛋白有助於檢測酪蛋白水解活性。 存在於培養基中的生色混合物被酶切割 β-葡萄糖苷酶導致綠藍色至藍色的菌落。 為了選擇性分離乳桿菌，加入環丙沙星補充劑（FD345），其抑制伴隨的細菌。
乳桿菌屬於革蘭氏陽性，兼性厭氧或微需氧桿狀桿狀非孢子形成細菌。 它們是乳酸菌群的主要部分。 隨著越來越多的LAB以混合形式開發和銷售為益生菌，有必要開發一種計算混合物中每種LAB的方法（1） 該培養基含有蛋白腖和HM提取物，提供含氮和含碳化合物，長鏈氨基酸，維生素和其他必需營養素。 甘露醇用作可發酵的碳水化合物，其發酵可以通過酚紅檢測。 M蛋白有助於檢測酪蛋白水解活性。 存在於培養基中的生色混合物被酶切割 β-葡萄糖苷酶導致綠藍色至藍色的菌落。 為了選擇性分離乳桿菌，加入環丙沙星補充劑（FD345），其抑制伴隨的細菌。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌，
HiMedia 顯色培養基產品 更多元，更豐富，品質優越。