HIMEDIA HiCrome 顯色培養基（／產色培養基／呈色培養基） |
顯色培養基（／產色培養基／呈色培養基）
( Chromogenic / Fluorogenic Culture Media )
顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
(一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形，
藉此直接觀察菌落顏色即可對菌種做出鑑定，
其反應的靈敏度和特異性大大優於傳統培養基。
顯色培養基通常為乾粉狀，容易儲存。
加蒸餾水溶解後，部分產品無須高壓滅菌，培養時間依具體培養基而定，
通常是18-24小時，比傳統時間顯著縮短。
HiMedia – M2064_HiCrome™ M-Coliconfirm Broth Base |
Recommended for detection of E.coli and other total coliforms in water samples by membrane filtration.
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Principle and Interpretation
This is a selective medium recommended for the simultaneous detection of Escherichia coli and total coliforms in water (1). The water sample is filtered through membranes and then placed on pad saturated with medium and incubated at 35 ±5°C for 24 hours in sealed Petri plates. Tryptone provides nitrogeneous and carbonaceous compounds, long chain amino acids, vitamins and other essential nutrients. Yeast extract serves as a source of vitamins. Lactose is the fermentable carbohydrate. The phosphates in the medium buffers the medium. Sodium chloride maintains the osmotic balance. The enzyme beta-glucuronidase produced by E.coli utilizes the chromogenic substrate to produce blue purple coloured colonies. Coliforms other than Escherichia coli turn red as they reduce TTC (2,3,5-triphenyl tetrazolium chloride). Thus, the resulting colour distinction allows simple interpretation of test without further confirmation. Methylene blue and ECC selective supplement containing imparts selectivity to the medium. Non-coliforms usually give white coloured colonies.
This is a selective medium recommended for the simultaneous detection of Escherichia coli and total coliforms in water (1). The water sample is filtered through membranes and then placed on pad saturated with medium and incubated at 35 ±5°C for 24 hours in sealed Petri plates. Tryptone provides nitrogeneous and carbonaceous compounds, long chain amino acids, vitamins and other essential nutrients. Yeast extract serves as a source of vitamins. Lactose is the fermentable carbohydrate. The phosphates in the medium buffers the medium. Sodium chloride maintains the osmotic balance. The enzyme beta-glucuronidase produced by E.coli utilizes the chromogenic substrate to produce blue purple coloured colonies. Coliforms other than Escherichia coli turn red as they reduce TTC (2,3,5-triphenyl tetrazolium chloride). Thus, the resulting colour distinction allows simple interpretation of test without further confirmation. Methylene blue and ECC selective supplement containing imparts selectivity to the medium. Non-coliforms usually give white coloured colonies.
原理與解釋
這是推薦用於同時檢測的選擇性培養基 大腸桿菌和水中的總大腸桿菌（1）。將水樣過濾通過膜，然後置於用培養基飽和的墊上，並在密封的培養皿中於35±5℃溫育24小時。 胰蛋白腖提供硝基和碳質化合物，長鏈氨基酸，維生素和其他必需營養素。酵母提取物是維生素的來源。乳糖是可發酵的碳水化合物。 培養基中的磷酸鹽緩衝培養基。氯化鈉保持滲透平衡。由大腸桿菌產生的酶β-葡醣醛酸糖苷酶利用顯色底物產生藍紫色的菌落。除大腸桿菌外的大腸桿菌在減少TTC（2,3,5-三苯基四唑氯化物）時變紅。因此，由此產生的顏色區分允許簡單地解釋測試而無需進一步確認。亞甲藍和ECC選擇性補充劑賦予培養基選擇性。非大腸菌群通常會產生白色菌落。
這是推薦用於同時檢測的選擇性培養基 大腸桿菌和水中的總大腸桿菌（1）。將水樣過濾通過膜，然後置於用培養基飽和的墊上，並在密封的培養皿中於35±5℃溫育24小時。 胰蛋白腖提供硝基和碳質化合物，長鏈氨基酸，維生素和其他必需營養素。酵母提取物是維生素的來源。乳糖是可發酵的碳水化合物。 培養基中的磷酸鹽緩衝培養基。氯化鈉保持滲透平衡。由大腸桿菌產生的酶β-葡醣醛酸糖苷酶利用顯色底物產生藍紫色的菌落。除大腸桿菌外的大腸桿菌在減少TTC（2,3,5-三苯基四唑氯化物）時變紅。因此，由此產生的顏色區分允許簡單地解釋測試而無需進一步確認。亞甲藍和ECC選擇性補充劑賦予培養基選擇性。非大腸菌群通常會產生白色菌落。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌，
HiMedia 顯色培養基產品 更多元，更豐富，品質優越。