HIMEDIA HiCrome 顯色培養基（／產色培養基／呈色培養基） |
顯色培養基（／產色培養基／呈色培養基）
( Chromogenic / Fluorogenic Culture Media )
顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
(一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形，
藉此直接觀察菌落顏色即可對菌種做出鑑定，
其反應的靈敏度和特異性大大優於傳統培養基。
顯色培養基通常為乾粉狀，容易儲存。
加蒸餾水溶解後，部分產品無須高壓滅菌，培養時間依具體培養基而定，
通常是18-24小時，比傳統時間顯著縮短。
HiMedia – M2062I HiCrome™ Cronobacter Isolation Agar (CCI Agar) |
Recommended for the isolation and identification of Cronobacter sakazakii from food products. The composition and performance of this media are as per specifications laid down in in ISO /TS 22964: 2017
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Principle and Interpretation
Enterobacter species are widely distributed in nature occurring in fresh water, soil, sewage, plants, vegetables, animal and human feaces. # Cronobacter sakazakii has been closely associated with neonatal meningitis and sepsis (1). HiCrome™ Cronobacter isolation Agar is recommended by ISO Committee for the isolation and identification of # C.sakazakii from food samples (2). The chromogenic substrate (5-Bromo-4-chloro-3-indolyl a-Dglucopyranoside) is cleaved specifically (3) by *C.sakazakii resulting in the formation of blue green colonies. Other organisms, which do not cleave this substrate, produce colourless coloured colonies. Tryptone and yeast extract provides nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other essential growth nutrients. Sodium chloride helps in maintaining the osmotic equilibrium of the medium. Sodium deoxycholate inhibits the accompanying gram-positive flora. Key: # : Formerly known as Enterobacter sakazakii.
Enterobacter species are widely distributed in nature occurring in fresh water, soil, sewage, plants, vegetables, animal and human feaces. # Cronobacter sakazakii has been closely associated with neonatal meningitis and sepsis (1). HiCrome™ Cronobacter isolation Agar is recommended by ISO Committee for the isolation and identification of # C.sakazakii from food samples (2). The chromogenic substrate (5-Bromo-4-chloro-3-indolyl a-Dglucopyranoside) is cleaved specifically (3) by *C.sakazakii resulting in the formation of blue green colonies. Other organisms, which do not cleave this substrate, produce colourless coloured colonies. Tryptone and yeast extract provides nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other essential growth nutrients. Sodium chloride helps in maintaining the osmotic equilibrium of the medium. Sodium deoxycholate inhibits the accompanying gram-positive flora. Key: # : Formerly known as Enterobacter sakazakii.
原理與解釋
腸桿菌屬物種在新鮮的自然界中廣泛分佈 水，土壤，污水，植物，蔬菜，動物和人類的糞便。 ＃ 阪崎腸桿菌與新生兒腦膜炎和敗血症密切相關（1）。 ISO委員會推薦使用HiCrome™Cronobacter分離瓊脂從食品樣品中分離和鑑定＃C.sakazakii（2）。 顯色底物（5-溴-4-氯-3-吲哚基a-Dglucopyranoside） 通過* C.sakazakii特異性地切割（3），導致藍綠色菌落的形成。 其他不切割該底物的生物會產生無色的菌落。 胰蛋白腖和酵母提取物提供含氮和含碳化合物，長鏈氨基酸，維生素和其他必需的生長營養素。 氯化鈉有助於維持培養基的滲透平衡。 脫氧膽酸鈉抑制伴隨的革蘭氏陽性菌群。 關鍵：＃ ：以前稱為阪崎腸桿菌。
腸桿菌屬物種在新鮮的自然界中廣泛分佈 水，土壤，污水，植物，蔬菜，動物和人類的糞便。 ＃ 阪崎腸桿菌與新生兒腦膜炎和敗血症密切相關（1）。 ISO委員會推薦使用HiCrome™Cronobacter分離瓊脂從食品樣品中分離和鑑定＃C.sakazakii（2）。 顯色底物（5-溴-4-氯-3-吲哚基a-Dglucopyranoside） 通過* C.sakazakii特異性地切割（3），導致藍綠色菌落的形成。 其他不切割該底物的生物會產生無色的菌落。 胰蛋白腖和酵母提取物提供含氮和含碳化合物，長鏈氨基酸，維生素和其他必需的生長營養素。 氯化鈉有助於維持培養基的滲透平衡。 脫氧膽酸鈉抑制伴隨的革蘭氏陽性菌群。 關鍵：＃ ：以前稱為阪崎腸桿菌。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌，
HiMedia 顯色培養基產品 更多元，更豐富，品質優越。