HIMEDIA HiCrome 顯色培養基（／產色培養基／呈色培養基） |
顯色培養基（／產色培養基／呈色培養基）
( Chromogenic / Fluorogenic Culture Media )
顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
(一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形，
藉此直接觀察菌落顏色即可對菌種做出鑑定，
其反應的靈敏度和特異性大大優於傳統培養基。
顯色培養基通常為乾粉狀，容易儲存。
加蒸餾水溶解後，部分產品無須高壓滅菌，培養時間依具體培養基而定，
通常是18-24小時，比傳統時間顯著縮短。
HiMedia – M2009 HiCrome™ L. mono Differential Agar Base |
Recommended for the selective and differential isolation, enumeration and identification of Listeria monocytogenes and Listeria species based on PCPLC activity .
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Principle and Interpretation
Listeria monocytogenes is a gram-positive foodborne human pathogen responsible for serious infections in pregnant women that may ultimately result in abortion, stillbirth, birth of a child with neonatal listeriosis and meningitis or primary bacteremia in adults and juveniles (2). The pathogenicity of Listeria ivanovii for humans is uncertain. Since L.monocytogenes and L.innocua have similar biochemical properties, they cannot be differentiated on traditional media (PALCAM, Oxford). HiCrome L.mono Differential Agar Base is based on for the selective and differential isolation of Listeria species on the basis of utilization of chromogenic substrate and lecithinase activity [Phosphotidylcholine hospholipase C (PCPLC)] (3). PI-PLC and PC-PLC, the major virulence factors, are only produced by pathogenic L. monocytogenes and Listeria ivanovii (1) Peptone, tryptone, yeast extract and sodium pyruvate provide nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and essential growth nutrients . Maltose is the fermentable carbohydrate. Sodium chloride maintains osmotic equilibrium. Phosphate buffers the medium. Lithium chloride and added selective supplement (FD333) inhibit accompanying microflora and allow the growth of Listeria species. Listeria species hydrolyse the chromogenic substrate and produces green coloured colonies. Lecithin solution (FD332) helps in detecting PCPLC activity. Differentiation of Listeria species is based on phosphatidylcholine phospholipase C (PCPLC) activity. L. monocytogenes and L.ivanovii exhibits PCPLC activity which is seen as opaque halo around the colony.
Listeria monocytogenes is a gram-positive foodborne human pathogen responsible for serious infections in pregnant women that may ultimately result in abortion, stillbirth, birth of a child with neonatal listeriosis and meningitis or primary bacteremia in adults and juveniles (2). The pathogenicity of Listeria ivanovii for humans is uncertain. Since L.monocytogenes and L.innocua have similar biochemical properties, they cannot be differentiated on traditional media (PALCAM, Oxford). HiCrome L.mono Differential Agar Base is based on for the selective and differential isolation of Listeria species on the basis of utilization of chromogenic substrate and lecithinase activity [Phosphotidylcholine hospholipase C (PCPLC)] (3). PI-PLC and PC-PLC, the major virulence factors, are only produced by pathogenic L. monocytogenes and Listeria ivanovii (1) Peptone, tryptone, yeast extract and sodium pyruvate provide nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and essential growth nutrients . Maltose is the fermentable carbohydrate. Sodium chloride maintains osmotic equilibrium. Phosphate buffers the medium. Lithium chloride and added selective supplement (FD333) inhibit accompanying microflora and allow the growth of Listeria species. Listeria species hydrolyse the chromogenic substrate and produces green coloured colonies. Lecithin solution (FD332) helps in detecting PCPLC activity. Differentiation of Listeria species is based on phosphatidylcholine phospholipase C (PCPLC) activity. L. monocytogenes and L.ivanovii exhibits PCPLC activity which is seen as opaque halo around the colony.
原理與解釋
單核細胞增生李斯特菌是一種革蘭氏陽性食源性人類病原體，可導致孕婦嚴重感染，最終可能導致流產，死產，新生兒李斯特菌病和腦膜炎或成人和青少年原發性菌血症的出生（2）。李斯特菌對人類的致病性尚不確定。由於單核細胞增生李斯特菌和L.innocua具有相似的生化特性，因此無法在傳統培養基（PALCAM，Oxford）上進行區分。 HiCrome L.mono差異瓊脂鹼基於利用顯色底物和卵磷脂酶活性[磷脂酰膽鹼磷脂酶C（PCPLC）]（3）對李斯特菌屬物種進行選擇性和差異分離。 PI-PLC和PC-PLC是主要的致病因子，僅由致病性單核細胞增生李斯特菌和李斯特菌（Iisterovii）產生（1）蛋白腖，胰蛋白腖，酵母提取物和丙酮酸鈉提供含氮和含碳化合物，長鏈氨基酸，維生素和必需品生長營養素。麥芽糖是可發酵的碳水化合物。氯化鈉保持滲透平衡。磷酸鹽緩衝介質。氯化鋰和添加的選擇性補充劑（FD333）抑制伴隨的微生物群並允許李斯特菌屬物種的生長。李斯特菌物種水解生色底物並產生綠色菌落。卵磷脂溶液（FD332）有助於檢測PCPLC活性。 李斯特菌物種的分化基於磷脂酰膽鹼磷脂酶C（PCPLC）活性。單核細胞增生李斯特氏菌和L.ivanovii表現出PCPLC活性，其被視為在菌落周圍的不透明暈圈。
單核細胞增生李斯特菌是一種革蘭氏陽性食源性人類病原體，可導致孕婦嚴重感染，最終可能導致流產，死產，新生兒李斯特菌病和腦膜炎或成人和青少年原發性菌血症的出生（2）。李斯特菌對人類的致病性尚不確定。由於單核細胞增生李斯特菌和L.innocua具有相似的生化特性，因此無法在傳統培養基（PALCAM，Oxford）上進行區分。 HiCrome L.mono差異瓊脂鹼基於利用顯色底物和卵磷脂酶活性[磷脂酰膽鹼磷脂酶C（PCPLC）]（3）對李斯特菌屬物種進行選擇性和差異分離。 PI-PLC和PC-PLC是主要的致病因子，僅由致病性單核細胞增生李斯特菌和李斯特菌（Iisterovii）產生（1）蛋白腖，胰蛋白腖，酵母提取物和丙酮酸鈉提供含氮和含碳化合物，長鏈氨基酸，維生素和必需品生長營養素。麥芽糖是可發酵的碳水化合物。氯化鈉保持滲透平衡。磷酸鹽緩衝介質。氯化鋰和添加的選擇性補充劑（FD333）抑制伴隨的微生物群並允許李斯特菌屬物種的生長。李斯特菌物種水解生色底物並產生綠色菌落。卵磷脂溶液（FD332）有助於檢測PCPLC活性。 李斯特菌物種的分化基於磷脂酰膽鹼磷脂酶C（PCPLC）活性。單核細胞增生李斯特氏菌和L.ivanovii表現出PCPLC活性，其被視為在菌落周圍的不透明暈圈。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌，
HiMedia 顯色培養基產品 更多元，更豐富，品質優越。