HiMedia – M1985 HiCrome Malassezia Agar (Twin Pack)

Principle and Interpretation
Malassezia is a genus of fungi, naturally found on the skin surfaces of many animals, including humans. Media based on malt extract is appreciated by many microbiologists due to their richness and nutrient balance especially for the cultivation of fastidious microorganisms. With acidic pH, they are used for the isolation, cultivation and maintenance of yeast and moulds. M. furfur is a lipophilic yeast, therefore in vitro growth must be stimulated by natural oils or other fatty substances. Peptone special provides nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other essential growth nutrients. Low pH favours fungal growth and inhibits contaminating bacteria from test samples (1).Tween 40, Glycerol monooleate enhances the growth of Malessezia species as it is a lipophilic yeast. Some pathogenic fungi may produce infective spores which are easily dispersed in air, so examination should be carried out in safety cabinet. For heavily contaminated samples, the plate must be supplemented with inhibitory agents for inhibiting bacterial growth with lower pH.

原理與解釋
馬拉色菌屬（Malassezia）是一種真菌，天然存在於許多動物（包括人類）的皮膚表面。基於麥芽提取物的培養基由於其豐富性和營養平衡而受到許多微生物學家的青睞，特別是對於苛刻微生物的培養。在酸性pH下，它們用於酵母和黴菌的分離，培養和維持。 M. furfur是一種親脂性酵母，因此體外生長必須受到天然油或其他脂肪物質的刺激。蛋白腖特別提供含氮和含碳化合物，長鏈氨基酸，維生素和其他必需的生長營養素。低pH有利於真菌生長並抑制來自測試樣品的污染細菌（1）。吐溫40，甘油單油酸酯增強了Malessezia物種的生長，因為它是親脂性酵母。一些致病真菌可能產生易於分散在空氣中的感染性孢子，因此應在安全櫃中進行檢查。對於嚴重污染的樣品，該板必須補充抑製劑，以抑制細菌生長，同時降低pH值。