HiMedia – M1974 HiCrome™ Rapid MRSA Agar Base

Principle and Interpretation
MRSA is a resistant variation of the common bacterium Staphylococcus aureus. It is an invasive pathogen that can cause disease in almost any tissue or organ in the human body, primarily in compromised individuals (1). Staphylococcal infections were earlier treated using Penicillin. But over the years resistance to this drug developed. Methicillin was the nextdrug of choice. While methicillin is very effective in treating most Staphylococcus infections some strains have developed resistance to methicillin and can no longer be killed by this antibiotic. These resistant bacteria are called Methicillin Resistant Staphylococcus aureus (MRSA) (2). Patients with breaks in their skin due to wound, indwelling catheters or burns are thosewith certain risk of developing MRSA infection (3). Special peptone, Casitose and amino-vitamin mix provides essential nutrients for growth. Carbohydrate is the source of carbon and energy. Phenol red is the pH indicator. The chromogenic mixture incorporated in the medium is specifically cleaved by Staphylococcus aureus (MRSA) to give greenish yellow coloured colonies. Sodium chloride in the medium helps to maintain the osmotic equilibrium of the medium. High concentration of sodium chloride also helps in inhibiting the accompanying microflora. Agar acts as solidifying agent.

原理與解釋
MRSA是常見細菌金黃色葡萄球菌的抗性變異。它是一種侵入性病原體，幾乎可以在人體的任何組織或器官中引起疾病​​，主要是在受損的個體中（1）。早期使用青黴素治療葡萄球菌感染。但多年來，這種藥物的耐藥性得到了發展。 甲氧西林是下一個選擇的藥物。雖然甲氧西林在治療大多數葡萄球菌感染方面非常有效，但一些菌株已經對甲氧西林產生抗藥性，並且不再能被這種抗生素殺死。這些抗性細菌被稱為耐甲氧西林金黃色葡萄球菌（MRSA）（2）。中斷患者 他們的皮膚由於傷口，留置導尿管或燒傷而具有一定的MRSA感染風險（3）。 特殊的蛋白腖，Casitose和氨基維生素混合物為生長提供必需的營養素。碳水化合物是碳和能量的來源。 酚紅是pH指示劑。摻入培養基中的生色混合物被金黃色葡萄球菌（MRSA）特異性切割，得到帶黃綠色的菌落。培養基中的氯化鈉有助於維持培養基的滲透平衡。高濃度的氯化鈉也有助於抑制伴隨的微生物群落。瓊脂充當凝固劑。