HiMedia – M1414andM1376 HiCrome™ Enterococci Agar / Broth

Principle and Interpretation
HiCrome™ Enterococci media are formulated on the basis of the work carried out by Althous et al (1), Amoras (2), Litsky et al (3), and Manafi and Sommer (4) and Snyder and Lichstein (5). These media are recommended for the rapid detection of Enterococci from water samples. The presence of Enterococcus group, which is a subgroup of the faecal streptococci, serves as a valuable bacterial indicator for determining the extent of faecal contamination (1, 6) and it is more specific than the detection of coliforms, which may originate from non-faecal sources. The enzyme b-glucosidase produced by Enterococci cleaves the chromogenic substrate, resulting in a bluish green colour. The medium contains peptone special, which provides nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other essential nutrients. Sodium chloride maintains the osmotic balance of the medium. Sodium azide inhibits the accompanying microflora, especially gram-negative organisms. Polysorbate 80 (Tween 80) acts as a source of fatty acids.

原理與解釋
HiCrome™腸球菌培養基是根據Althous等人（1），Amoras（2），Litsky等人（3），Manafi和Sommer（4）以及Snyder和Lichstein（5）開展的工作制定的。建議將這些培養基用於從水樣中快速檢測腸球菌。腸球菌群的存在，是糞鏈球菌的一個亞群，是一種有價值的細菌指示劑。 確定糞便污染的程度（1,6），它比檢測大腸菌更具特異性，大腸菌可能來自非糞便來源。由腸球菌產生的酶β-葡萄糖苷酶切割生色底物，產生藍綠色。 該培養基含有特殊的蛋白腖，可提供含氮和含碳化合物，長鏈氨基酸，維生素和其他必需營養素。氯化鈉維持培養基的滲透平衡。疊氮化鈉抑制伴隨的微生物區系，尤其是革蘭氏陰性菌。聚山梨醇酯80（吐溫80）充當脂肪酸的來源。