HIMEDIA HiCrome 顯色培養基（／產色培養基／呈色培養基） |
顯色培養基（／產色培養基／呈色培養基）
( Chromogenic / Fluorogenic Culture Media )
顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
(一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形，
藉此直接觀察菌落顏色即可對菌種做出鑑定，
其反應的靈敏度和特異性大大優於傳統培養基。
顯色培養基通常為乾粉狀，容易儲存。
加蒸餾水溶解後，部分產品無須高壓滅菌，培養時間依具體培養基而定，
通常是18-24小時，比傳統時間顯著縮短。
HiMedia – M1078andM1082 Salmonella Differential Agar / Modified (Twin pack) (RajHans Medium) |
Recommended for identification and differentiation of Salmonella species from members of Enterobacteriaceae, especially Proteus species.
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Principle and Interpretation
Salmonella Differential Agar media are slight modification of original formulation of Rambach (3) used for differentiation of Salmonella species from Proteus species and other enteric bacteria. Production of acid from propylene glycol is a novel characteristic of Salmonella species and is utilized in these media. Many of the media such as SS Agar, XLD Agar recommended for the identification and differentiation of Salmonella species (1) are based on lactose fermentation and hydrogen sulphide production. Peptone special and yeast extract provides nitrogenous and carbonaceous compounds, long chain amino acid, vitamins and other essential growth nutrients. Sodium deoxycholate inhibits gram-positive organisms rendering the medium selective for enteric microorganisms. The BC indicator turns pink in presence of acid produced from propylene glycol. Lactose fermenting ability is determined by using an indicator, which can detect the presence of enzyme b-galactosidase. Lactose fermenting (b-galactosidase producing) bacteria yield blue violet coloured colony (2). Salmonellae produce acid from propylene glycol and on combining with the BC indicator gives typical pink red colonies. Other enteric gram-negative bacteria form colourless colonies. Salmonella Typhimurium and Salmonella Enteritidis produce pink to red colonies. Specimen should be enriched in an appropriate selective enrichment broth. This enriched culture is then inoculated on SalmonellaDifferential Agar/ Salmonella Differential Agar, Modified and incubated at 35-37°C for 24-48 hours.
Salmonella Differential Agar media are slight modification of original formulation of Rambach (3) used for differentiation of Salmonella species from Proteus species and other enteric bacteria. Production of acid from propylene glycol is a novel characteristic of Salmonella species and is utilized in these media. Many of the media such as SS Agar, XLD Agar recommended for the identification and differentiation of Salmonella species (1) are based on lactose fermentation and hydrogen sulphide production. Peptone special and yeast extract provides nitrogenous and carbonaceous compounds, long chain amino acid, vitamins and other essential growth nutrients. Sodium deoxycholate inhibits gram-positive organisms rendering the medium selective for enteric microorganisms. The BC indicator turns pink in presence of acid produced from propylene glycol. Lactose fermenting ability is determined by using an indicator, which can detect the presence of enzyme b-galactosidase. Lactose fermenting (b-galactosidase producing) bacteria yield blue violet coloured colony (2). Salmonellae produce acid from propylene glycol and on combining with the BC indicator gives typical pink red colonies. Other enteric gram-negative bacteria form colourless colonies. Salmonella Typhimurium and Salmonella Enteritidis produce pink to red colonies. Specimen should be enriched in an appropriate selective enrichment broth. This enriched culture is then inoculated on SalmonellaDifferential Agar/ Salmonella Differential Agar, Modified and incubated at 35-37°C for 24-48 hours.
原理與解釋
沙門氏菌差異瓊脂培養基是Rambach（3）原始配方的輕微修飾，用於從變形桿菌屬物種和其他腸道細菌中分化沙門氏菌。從丙二醇生產酸是沙門氏菌物種的新特徵，並用於這些培養基中。 SS Agar，XLD瓊脂等許多媒介被推薦用於鑑定和分化沙門氏菌（1），它們基於乳糖發酵和硫化氫生產。 蛋白腖特殊和酵母提取物提供含氮和含碳化合物，長鏈氨基酸，維生素和其他必需的生長營養素。脫氧膽酸鈉抑制革蘭氏陽性菌，使培養基對腸道微生物具有選擇性。 BC指示劑在丙二醇產生的酸存在下變為粉紅色。通過使用指示劑確定乳糖發酵能力，所述指示劑可以檢測酶b-半乳糖苷酶的存在。 乳糖發酵（產生β-半乳糖苷酶）細菌產生藍紫色菌落（2）。沙門氏菌從丙二醇中產生酸，並與BC指示劑結合，產生典型的粉紅色菌落。其他腸道革蘭氏陰性細菌形成無色菌落。鼠傷寒沙門氏菌和腸炎沙門氏菌產生粉紅色到紅色的菌落。應在適當的選擇性富集肉湯中富集標本。然後將該富集的培養物接種在SalmonellaDiffere Agar / Salmonella Differential Agar上，修飾並在35-37℃溫育24-48小時。
沙門氏菌差異瓊脂培養基是Rambach（3）原始配方的輕微修飾，用於從變形桿菌屬物種和其他腸道細菌中分化沙門氏菌。從丙二醇生產酸是沙門氏菌物種的新特徵，並用於這些培養基中。 SS Agar，XLD瓊脂等許多媒介被推薦用於鑑定和分化沙門氏菌（1），它們基於乳糖發酵和硫化氫生產。 蛋白腖特殊和酵母提取物提供含氮和含碳化合物，長鏈氨基酸，維生素和其他必需的生長營養素。脫氧膽酸鈉抑制革蘭氏陽性菌，使培養基對腸道微生物具有選擇性。 BC指示劑在丙二醇產生的酸存在下變為粉紅色。通過使用指示劑確定乳糖發酵能力，所述指示劑可以檢測酶b-半乳糖苷酶的存在。 乳糖發酵（產生β-半乳糖苷酶）細菌產生藍紫色菌落（2）。沙門氏菌從丙二醇中產生酸，並與BC指示劑結合，產生典型的粉紅色菌落。其他腸道革蘭氏陰性細菌形成無色菌落。鼠傷寒沙門氏菌和腸炎沙門氏菌產生粉紅色到紅色的菌落。應在適當的選擇性富集肉湯中富集標本。然後將該富集的培養物接種在SalmonellaDiffere Agar / Salmonella Differential Agar上，修飾並在35-37℃溫育24-48小時。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌，
HiMedia 顯色培養基產品 更多元，更豐富，品質優越。