HIMEDIA HiCrome 顯色培養基（／產色培養基／呈色培養基） |
顯色培養基（／產色培養基／呈色培養基）
( Chromogenic / Fluorogenic Culture Media )
顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
(一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形，
藉此直接觀察菌落顏色即可對菌種做出鑑定，
其反應的靈敏度和特異性大大優於傳統培養基。
顯色培養基通常為乾粉狀，容易儲存。
加蒸餾水溶解後，部分產品無須高壓滅菌，培養時間依具體培養基而定，
通常是18-24小時，比傳統時間顯著縮短。
M1293_HiCrome™ ECC Agar |
Recommended as a differential medium for presumptive identification of Escherichia coli and other coliforms in food and environmental samples.
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Principle and Interpretation
Escherichia coli, a member of the family Enterobacteriaceae is a part of normal flora of the intestinal tract of humans and a variety of animals. Although most of E. coli does not cause gastrointestinal illnesses, certain groups of E. coli can cause life-threatening diarrhoea and severe sequelae or disability (1). HiCrome™ ECC Agar is a differential medium recommended for the presumptive identification of E. coli and other coliforms in food and environmental samples (2). The medium contains two chromogens. One of the chromogen is cleaved by the enzyme b-glucuronidase produced by E. coli to give blue to purple coloured colonies whereas the other chromogen is cleaved by the enzyme galactosidase, produced by majority of coliforms, resulting in the formation of rose-pink coloured colonies (3, 4). Peptone special, yeast extract provide nitrogenous substances, carbonaceous compounds, long chain amino acids, vitamin B complex and other essential growth nutrients. Lactose is the fermentable carbohydrate, which aids in detecting lactose fermenters with neutral red as an indicator. Disodium hydrogen phosphate and potassium dihydrogen phosphate buffers the medium well. Sodium chloride maintains the osmotic equilibrium. Dry the surface of plate medium before use. Dilute the food sample 1: 5 or 1: 10 with 0.1% sterile Peptone Water (M028) and homogenize in a blender or a stomacher. Spread 0.5 ml or 1.0 ml of the homogenate over the agar surface with a sterile glass spreader and incubate the plates at 35-37°C for 18-24 hours. Count the blue/purple colonies and multiply with the dilution factor. The number of E. coli is reported per gram of food. The medium should be used only for in-vitro diagnostic purpose. Wear mask while handling the dehydrated product and avoid contact with eyes.
Escherichia coli, a member of the family Enterobacteriaceae is a part of normal flora of the intestinal tract of humans and a variety of animals. Although most of E. coli does not cause gastrointestinal illnesses, certain groups of E. coli can cause life-threatening diarrhoea and severe sequelae or disability (1). HiCrome™ ECC Agar is a differential medium recommended for the presumptive identification of E. coli and other coliforms in food and environmental samples (2). The medium contains two chromogens. One of the chromogen is cleaved by the enzyme b-glucuronidase produced by E. coli to give blue to purple coloured colonies whereas the other chromogen is cleaved by the enzyme galactosidase, produced by majority of coliforms, resulting in the formation of rose-pink coloured colonies (3, 4). Peptone special, yeast extract provide nitrogenous substances, carbonaceous compounds, long chain amino acids, vitamin B complex and other essential growth nutrients. Lactose is the fermentable carbohydrate, which aids in detecting lactose fermenters with neutral red as an indicator. Disodium hydrogen phosphate and potassium dihydrogen phosphate buffers the medium well. Sodium chloride maintains the osmotic equilibrium. Dry the surface of plate medium before use. Dilute the food sample 1: 5 or 1: 10 with 0.1% sterile Peptone Water (M028) and homogenize in a blender or a stomacher. Spread 0.5 ml or 1.0 ml of the homogenate over the agar surface with a sterile glass spreader and incubate the plates at 35-37°C for 18-24 hours. Count the blue/purple colonies and multiply with the dilution factor. The number of E. coli is reported per gram of food. The medium should be used only for in-vitro diagnostic purpose. Wear mask while handling the dehydrated product and avoid contact with eyes.
原理與解釋
大腸埃希氏菌是腸桿菌科的一員之一 , 人體腸道的正常菌群和各種動物。 雖然大多數大腸桿菌不會引起胃腸道疾病，但某些大腸桿菌可引起危及生命的腹瀉和嚴重的後遺症或殘疾（1）。 HiCrome™ECC瓊脂是推薦用於食品和環境樣品中大腸桿菌和其他大腸桿菌的推定鑑定的差異培養基（2）。該培養基含有兩種色原。其中一種色原被大腸桿菌產生的β-葡萄醣醛酸酶切割，產生藍色至紫色的菌落，而另一種色原被半乳糖苷酶切割，由大多數大腸桿菌產生，導致形成玫瑰色粉紅色殖民地（3,4）。 蛋白腖特殊，酵母提取物提供含氮物質，含碳化合物，長鏈氨基酸，維生素B複合物和其他必需的生長營養素。乳糖是可發酵的碳水化合物，有助於檢測以中性紅為指示劑的乳糖發酵罐。磷酸氫二鈉和磷酸二氫鉀緩衝培養基。氯化鈉保持滲透平衡。使用前擦乾平板介質表面。 用0.1％無菌蛋白腖水（M028）稀釋食物樣品1：5或1:10，並在攪拌器或stomacher中均化。用無菌玻璃塗佈器在瓊脂表面上鋪0.5ml或1.0ml勻漿，並在35-37℃下孵育平板18-24小時。計算藍色/紫色菌落並乘以稀釋倍數。每克食物報告大腸桿菌的數量。培養基應僅用於體外診斷目的。處理脫水產品時戴口罩，避免接觸眼睛。
大腸埃希氏菌是腸桿菌科的一員之一 , 人體腸道的正常菌群和各種動物。 雖然大多數大腸桿菌不會引起胃腸道疾病，但某些大腸桿菌可引起危及生命的腹瀉和嚴重的後遺症或殘疾（1）。 HiCrome™ECC瓊脂是推薦用於食品和環境樣品中大腸桿菌和其他大腸桿菌的推定鑑定的差異培養基（2）。該培養基含有兩種色原。其中一種色原被大腸桿菌產生的β-葡萄醣醛酸酶切割，產生藍色至紫色的菌落，而另一種色原被半乳糖苷酶切割，由大多數大腸桿菌產生，導致形成玫瑰色粉紅色殖民地（3,4）。 蛋白腖特殊，酵母提取物提供含氮物質，含碳化合物，長鏈氨基酸，維生素B複合物和其他必需的生長營養素。乳糖是可發酵的碳水化合物，有助於檢測以中性紅為指示劑的乳糖發酵罐。磷酸氫二鈉和磷酸二氫鉀緩衝培養基。氯化鈉保持滲透平衡。使用前擦乾平板介質表面。 用0.1％無菌蛋白腖水（M028）稀釋食物樣品1：5或1:10，並在攪拌器或stomacher中均化。用無菌玻璃塗佈器在瓊脂表面上鋪0.5ml或1.0ml勻漿，並在35-37℃下孵育平板18-24小時。計算藍色/紫色菌落並乘以稀釋倍數。每克食物報告大腸桿菌的數量。培養基應僅用於體外診斷目的。處理脫水產品時戴口罩，避免接觸眼睛。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌，
HiMedia 顯色培養基產品 更多元，更豐富，品質優越。