HiMedia – M2026 HiCrome™ Clostridial Agar Base

Principle and Interpretation
One of the major species of anaerobic bacteria to cause disease in humans is Clostridium. Clostridium species cause tetanus and gas gangrene that ultimately leads to tissue damage. Another Clostridium species produces the lethal botulinum toxin, the causative agent of botulism (1). Clostridial Agar formulated by Vera is recommended for the selective isolation of pathogenic Clostridia form mixed flora (2). HiCrome is the modification for chromogenic differentiation. Tryptone provide the essential nutrients, mainly the nitrogen compounds. Yeast extract serves as source of vitamins esecially of the B group. Dextrose acts as fermentable carbohydrate source. Sodium thioglycollate is the reducing agents that help to create low oxidationreduction potential enabling the growth of Clostridia. Also the media is well supplemented to support luxuriant growth of Clostridium species. The selective supplements inhibits other enteric bacteria. The ideal method of inoculation of Clostridial Agar is direct inoculation of sterile, cooled medium with the specimen (in tubes). Alternatively agar plates of the medium can also be inoculated by streaking.

原理與解釋
梭菌（Clostridium）是導致人類疾病的厭氧細菌的主要種類之一。梭菌屬物種會導致破傷風和氣性壞疽，最終導致組織損傷。另一種Clostridium物種產生致命的肉毒桿菌毒素，肉毒桿菌中毒的致病因子（1）。由Vera配製的梭菌瓊脂被推薦用於從混合菌群中選擇性分離致病性梭菌（2）。 HiCrome是顯色分化的修飾。 胰蛋白腖提供必需的營養素，主要是氮化合物。酵母提取物作為B族維生素的來源。葡萄糖可作為可發酵的碳水化合物來源。巰基乙酸鈉是還原劑，有助於產生低氧化還原潛力，從而促進梭菌的生長。此外，媒體得到很好的補充，以支持梭菌屬物種的繁茂生長。 選擇性補充劑抑制其他腸道細菌。 接種梭菌瓊脂的理想方法是用樣品（在管中）直接接種無菌冷卻培養基。或者，培養基的瓊脂平板也可以通過劃線接種。