HiMedia – M2010 HiCrome™ Mueller Hinton Agar

Principle and Interpretation
The Mueller Hinton formulation was originally developed for the cultivation of pathogenic Neisseria species (1). Other media were subsequently developed that replaced the use of Mueller Hinton Agar for the cultivation of pathogenic Neisseria species, but it became widely used in the determination of sulfonamide resistance of gonococci and other organisms. Mueller Hinton Agar is now used as a test medium for antimicrobial susceptibility testing (2). Acicase provide nitrogenous compounds, carbon, sulphur and other essential nutrients. These ingredients are selected for low thymine and thymidine content as determined by MIC values for Enterococcus faecalis with sulfamethoxazole trimethoprim (SXT). Chromogenic mixture incorporated helps in colour differentiation. One of the chromogenic substrate is cleaved by b-glucosidase possessed by Enterococci resulting in formation of blue colonies. E.coli produce pink to purple colonies due to the enzyme b-D-galactosidase that cleaves the other chromogenic substrate. Staphylococcus aureus produces colourless colonies. Pseudomonas aeruginosa produces greenish pigmentation. Klebsiella and Enterobacter species produces metallic blue colured colonies. Colonies of Proteus, Morganella and Providencia species appear brown .This medium can be employed in screening urinary tract pathogens wherein organisms can be differentiated based on colour and simultaneously the antibiotic sensitivity can be determined.

原理與解釋
Mueller Hinton配方最初是為培養致病性奈瑟氏菌種而開發的（1）。隨後開發了其他培養基，取代了Mueller Hinton瓊脂用於培養致病性奈瑟菌屬物種的用途，但它被廣泛用於測定淋球菌和其他生物體的磺酰胺抗性。 Mueller Hinton Agar現在被用作抗菌藥敏試驗的測試介質（2）。 Acicase提供含氮化合物，碳，硫和其他必需營養素。選擇這些成分用於低胸腺嘧啶和胸苷含量，如通過糞腸球菌與磺胺甲噁唑甲氧芐啶（SXT）的MIC值所確定的。摻入的顯色混合物有助於顏色分化。其中一個生色底物被腸球菌所具有的β-葡糖苷酶切割，導致藍色菌落的形成。由於b-D-半乳糖苷酶切割其他生色底物，大腸桿菌產生粉紅色到紫色的菌落。金黃色葡萄球菌產生無色菌落。銅綠假單胞菌產生綠色色素沉著。 克雷伯氏菌和腸桿菌屬物種產生金屬藍色的菌落。 Proteus，Morganella和Providencia物種的菌落顯示為褐色。該培養基可用於篩選尿路病原體，其中可以基於顏色區分生物體，同時可以確定抗生素敏感性。