HiMedia – M1960 HiCrome™ Bifidobacterium Agar

Principle and Interpretation
The genus Bifidobacterium is the third most numerous bacterial populations found in the human intestine after Bacteroides and Eubacterium. It is an anaerobic bacteria that makes up the gut microbial flora. It resides in the colon and have health benefits for their hosts. Bifidobacteria are also associated with lower incidences of allergies (1, 2). Bifidobacterium Agar isused for the cultivation and maintenance of Bifidobacterium species (3). Peptone special provides nitrogeneous and carbanaceous compounds, long chain amino acids, vitamins and other essential growth nutrients. Sodium chloride maintains osmotic balance. M Protein powder aids in detecting casein hydrolysis activity which is exhibited by Bifidobacterium breve. A halo zone is observed around the colony in case of casein hydrolysis. The indicator system in the chromogenic mixture helps in distinguishing between Lactobacillus and Bifidobacterium species. Lactobacillus species usually produce green colonies with opaque background. Bifidobacterium infantis produces dark blue to bluish green colonies. Agar serves as an solidifying agent.

原理與解釋
雙歧桿菌屬是在擬桿菌和真細菌之後在人腸中發現的第三大數量的細菌群。它是一種厭氧細菌，構成腸道微生物菌群。它存在於結腸中，對宿主有益。 雙歧桿菌也與較低的過敏發生率相關（1,2）。雙歧桿菌瓊脂用於雙歧桿菌屬的培養和維持（3）。 蛋白腖特別提供硝基和碳水化合物，長鏈氨基酸，維生素和其他必需的生長營養素。氯化鈉保持滲透平衡。 M蛋白粉有助於檢測酪短蛋白水解活性，這是由短雙歧桿菌（Bifidobacterium breve）表現出來的。在酪蛋白水解的情況下，在菌落周圍觀察到暈圈區。顯色混合物中的指示系統有助於區分乳桿菌屬和雙歧桿菌屬。乳桿菌屬物種通常產生具有不透明背景的綠色菌落。嬰兒雙歧桿菌產生深藍色至藍綠色菌落。瓊脂用作固化劑。