HIMEDIA HiCrome 顯色培養基（／產色培養基／呈色培養基） |
顯色培養基（／產色培養基／呈色培養基）
( Chromogenic / Fluorogenic Culture Media )
顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
(一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形，
藉此直接觀察菌落顏色即可對菌種做出鑑定，
其反應的靈敏度和特異性大大優於傳統培養基。
顯色培養基通常為乾粉狀，容易儲存。
加蒸餾水溶解後，部分產品無須高壓滅菌，培養時間依具體培養基而定，
通常是18-24小時，比傳統時間顯著縮短。
HiMedia – M1571M1713_ HiCrome™ M-TEC Agar AND HiCrome M-TEC Broth |
Recommended by the U.S. Environmental Protection Agency (USEPA) for differentiation and enumeration of thermotolerant Escherichia coli in water by the membrane filtration technique.
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Principle and Interpretation
HiCrome™ M-TEC Agar/Broth are the chromogenic media used for detection and enumeration of thermotolerant Escherichia coli (TEC) in water by membrane filtration (2). HiCrome™ M-TEC Broth is a modification of the M-TEC Agar developed by Dufour (1). The modified medium contains the chromogen, 5-bromo-6-chloro-3-indolyl-b-Dglucuronide that is cleaved by enzyme b-D-glucuronidase to yield glucuronic acid, produced by E. coli strains. This imparts a purplemagenta colour to the colonies of E. coli only. Proteose peptone and yeast extract provides nitrogenous carbonaceous compounds, amino acids and long chain peptides for the growth of microoganisms. Lactose is the fermentable carbohydrate. Sodium chloride maintains osmotic equilibrium. Potassium dihydrogen phosphate and dipotassium phosphate provide strong buffering system to control the pH in the presence of fermentative action. Sodium lauryl sulphate and sodium deoxycholate make the medium more selective by inhibiting gram-positive bacteria. Saturate a sterile cotton absorbent pad with about 2ml of HiCrome™ M-TEC Broth (M1713). Membrane filter through which water sample has been passed is aseptically placed on the saturated absorbent cotton pad face upwards. This absorbent pad is then incubated at 44.5 ± 0.2°C for 22-24 hours. Following incubation E. coli will form purple to magenta coloured colonies on the membrane filters.
HiCrome™ M-TEC Agar/Broth are the chromogenic media used for detection and enumeration of thermotolerant Escherichia coli (TEC) in water by membrane filtration (2). HiCrome™ M-TEC Broth is a modification of the M-TEC Agar developed by Dufour (1). The modified medium contains the chromogen, 5-bromo-6-chloro-3-indolyl-b-Dglucuronide that is cleaved by enzyme b-D-glucuronidase to yield glucuronic acid, produced by E. coli strains. This imparts a purplemagenta colour to the colonies of E. coli only. Proteose peptone and yeast extract provides nitrogenous carbonaceous compounds, amino acids and long chain peptides for the growth of microoganisms. Lactose is the fermentable carbohydrate. Sodium chloride maintains osmotic equilibrium. Potassium dihydrogen phosphate and dipotassium phosphate provide strong buffering system to control the pH in the presence of fermentative action. Sodium lauryl sulphate and sodium deoxycholate make the medium more selective by inhibiting gram-positive bacteria. Saturate a sterile cotton absorbent pad with about 2ml of HiCrome™ M-TEC Broth (M1713). Membrane filter through which water sample has been passed is aseptically placed on the saturated absorbent cotton pad face upwards. This absorbent pad is then incubated at 44.5 ± 0.2°C for 22-24 hours. Following incubation E. coli will form purple to magenta coloured colonies on the membrane filters.
原理與解釋
HiCrome™M-TEC瓊脂/肉湯是用於通過膜過濾檢測和計數水中耐熱大腸桿菌（TEC）的顯色培養基（2）。 HiCrome™M-TEC肉湯是Dufour（1）開發的M-TEC瓊脂的改良劑。改良培養基含有色原，5-溴-6-氯-3-吲哚基-b-葡糖苷酸，其被酶b-D-葡醣醛酸酶切割產生 葡萄醣醛酸，由大腸桿菌菌株產生。這僅賦予大腸桿菌菌落紫色的紫色。 蛋白腖和酵母提取物提供含氮碳質化合物，氨基酸和長鏈肽，用於微生物的生長。乳糖是可發酵的碳水化合物。氯化鈉保持滲透平衡。磷酸二氫鉀和磷酸二鉀提供強烈的緩衝系統以在發酵作用下控制pH。十二烷基硫酸鈉和脫氧膽酸鈉通過抑制革蘭氏陽性細菌使培養基更具選擇性。用約2ml HiCrome™M-TEC肉湯（M1713）浸透無菌棉吸水墊。將水樣通過的膜過濾器無菌地置於飽和的吸收棉墊面朝上。然後將該吸收墊在44.5±0.2℃下溫育22-24小時。孵育後，大腸桿菌將在膜過濾器上形成紫色至品紅色的菌落。
HiCrome™M-TEC瓊脂/肉湯是用於通過膜過濾檢測和計數水中耐熱大腸桿菌（TEC）的顯色培養基（2）。 HiCrome™M-TEC肉湯是Dufour（1）開發的M-TEC瓊脂的改良劑。改良培養基含有色原，5-溴-6-氯-3-吲哚基-b-葡糖苷酸，其被酶b-D-葡醣醛酸酶切割產生 葡萄醣醛酸，由大腸桿菌菌株產生。這僅賦予大腸桿菌菌落紫色的紫色。 蛋白腖和酵母提取物提供含氮碳質化合物，氨基酸和長鏈肽，用於微生物的生長。乳糖是可發酵的碳水化合物。氯化鈉保持滲透平衡。磷酸二氫鉀和磷酸二鉀提供強烈的緩衝系統以在發酵作用下控制pH。十二烷基硫酸鈉和脫氧膽酸鈉通過抑制革蘭氏陽性細菌使培養基更具選擇性。用約2ml HiCrome™M-TEC肉湯（M1713）浸透無菌棉吸水墊。將水樣通過的膜過濾器無菌地置於飽和的吸收棉墊面朝上。然後將該吸收墊在44.5±0.2℃下溫育22-24小時。孵育後，大腸桿菌將在膜過濾器上形成紫色至品紅色的菌落。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌，
HiMedia 顯色培養基產品 更多元，更豐富，品質優越。