HIMEDIA HiCrome 顯色培養基（／產色培養基／呈色培養基） |
顯色培養基（／產色培養基／呈色培養基）
( Chromogenic / Fluorogenic Culture Media )
顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
(一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形，
藉此直接觀察菌落顏色即可對菌種做出鑑定，
其反應的靈敏度和特異性大大優於傳統培養基。
顯色培養基通常為乾粉狀，容易儲存。
加蒸餾水溶解後，部分產品無須高壓滅菌，培養時間依具體培養基而定，
通常是18-24小時，比傳統時間顯著縮短。
HiMedia – M1488 HiCrome™ ECD Agar w/ MUG |
Recommended for the detection of Escherichia coli in water and food samples by using a combination of chromogenic and fluorogenic substrate.
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Principle and Interpretation
HiCrome™ ECD Agar w/ MUG is recommended for rapid detection of Escherichia coli by using a combination of chromogenic and fluorogenic substrates. The presence of Escherichia coli is indicated by blue coloured colony formation due to cleavage of chromogenic substrate. Fluorogenic substrate permits rapid detection of Escherichia coli when medium is observed for fluorescence using UV light (1, 2). Fluorogenic substrate also detects anaerogenic strains, which may not be detected in conventional procedure (1). It is hydrolysed by enzyme b-Dglucuronidase, possessed by Escherichia coli to yield a fluorescent end product. The reaction is indicated by a blue fluorescence under UV light. Tryptone provides nitrogenous, carbonaceous compounds, long chain amino acids and other essential nutrients. Lactose is the fermentable carbohydrate. Sodium chloride maintains osmotic equilibrium. The medium has a strong buffering system to control the pH in the presence of fermentive action. The bile salt mixture inhibits gram-positive bacteria especially Bacillus species and faecal Streptococci.
HiCrome™ ECD Agar w/ MUG is recommended for rapid detection of Escherichia coli by using a combination of chromogenic and fluorogenic substrates. The presence of Escherichia coli is indicated by blue coloured colony formation due to cleavage of chromogenic substrate. Fluorogenic substrate permits rapid detection of Escherichia coli when medium is observed for fluorescence using UV light (1, 2). Fluorogenic substrate also detects anaerogenic strains, which may not be detected in conventional procedure (1). It is hydrolysed by enzyme b-Dglucuronidase, possessed by Escherichia coli to yield a fluorescent end product. The reaction is indicated by a blue fluorescence under UV light. Tryptone provides nitrogenous, carbonaceous compounds, long chain amino acids and other essential nutrients. Lactose is the fermentable carbohydrate. Sodium chloride maintains osmotic equilibrium. The medium has a strong buffering system to control the pH in the presence of fermentive action. The bile salt mixture inhibits gram-positive bacteria especially Bacillus species and faecal Streptococci.
原理與解釋
建議將HiCrome™ECD Agar w / MUG用於通過使用生色和熒光底物的組合快速檢測大腸桿菌。由於髮色底物的裂解，藍色菌落形成表明存在大腸桿菌。 當使用UV光觀察培養基的熒光時，熒光底物允許快速檢測大腸桿菌（1,2）。熒光底物也檢測到致氣性菌株，這在傳統方法（1）中可能檢測不到。它被大腸桿菌所具有的酶b-Dglucuronidase水解，產生熒光終產物。在UV光下通過藍色熒光指示反應。 胰蛋白腖提供含氮，含碳化合物，長鏈氨基酸和其他必需營養素。乳糖是可發酵的碳水化合物。氯化鈉保持滲透平衡。該培養基具有強烈的緩衝系統，以在存在發酵作用的情況下控制pH。膽汁鹽混合物抑制革蘭氏陽性細菌，特別是芽孢桿菌屬和糞便鏈球菌。
建議將HiCrome™ECD Agar w / MUG用於通過使用生色和熒光底物的組合快速檢測大腸桿菌。由於髮色底物的裂解，藍色菌落形成表明存在大腸桿菌。 當使用UV光觀察培養基的熒光時，熒光底物允許快速檢測大腸桿菌（1,2）。熒光底物也檢測到致氣性菌株，這在傳統方法（1）中可能檢測不到。它被大腸桿菌所具有的酶b-Dglucuronidase水解，產生熒光終產物。在UV光下通過藍色熒光指示反應。 胰蛋白腖提供含氮，含碳化合物，長鏈氨基酸和其他必需營養素。乳糖是可發酵的碳水化合物。氯化鈉保持滲透平衡。該培養基具有強烈的緩衝系統，以在存在發酵作用的情況下控制pH。膽汁鹽混合物抑制革蘭氏陽性細菌，特別是芽孢桿菌屬和糞便鏈球菌。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌，
HiMedia 顯色培養基產品 更多元，更豐富，品質優越。