HIMEDIA HiCrome 顯色培養基（／產色培養基／呈色培養基） |
顯色培養基（／產色培養基／呈色培養基）
( Chromogenic / Fluorogenic Culture Media )
顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
(一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形，
藉此直接觀察菌落顏色即可對菌種做出鑑定，
其反應的靈敏度和特異性大大優於傳統培養基。
顯色培養基通常為乾粉狀，容易儲存。
加蒸餾水溶解後，部分產品無須高壓滅菌，培養時間依具體培養基而定，
通常是18-24小時，比傳統時間顯著縮短。
HiMedia – M1469 HiFluoro™ Pseudomonas Agar Base |
Recommended for selective isolation of Pseudomonas aeruginosa from clinical and non-clinical specimens by fluorogenic method.
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Principle and Interpretation
Pseudomonas aeruginosa (also known as Pseudomonas pyocyanea) is a gram-negative, aerobic, rod-shaped bacterium. Like other Pseudomonads, P. aeruginosa secretes a variety of pigments, including pyocyanin (blue-green), fluorescein (yellow-green and fluorescent), and pyorubin (red-brown). King et al developed Pseudomonas Agar P(i.e. King A media) for enhancing pyocyanin and pyorubin production and Pseudomonas Agar F (i.e. King B media) for enhancing fluorescein production (1). HiFluoro Pseudomonas Agar Base is devised based on the formula described by King et al. (1) except fluorogenic mixture. It is used as the selective medium for the isolation of P. aeruginosa from pus, sputum and drains etc.Cetrimide (Cetyltrimethylammonium bromide) is incorporated in the medium to inhibit bacteria other than P. aeruginosa. It acts as a quaternary ammonium compound, cationic detergent that causes nitrogen and phosphorus to be released from bacterial cells other than P. aeruginosa. P. aeruginosa cleaves the fluorogenic compound to release the fluorogen which produces a visible fluorescence under long wave UV light.
Pseudomonas aeruginosa (also known as Pseudomonas pyocyanea) is a gram-negative, aerobic, rod-shaped bacterium. Like other Pseudomonads, P. aeruginosa secretes a variety of pigments, including pyocyanin (blue-green), fluorescein (yellow-green and fluorescent), and pyorubin (red-brown). King et al developed Pseudomonas Agar P(i.e. King A media) for enhancing pyocyanin and pyorubin production and Pseudomonas Agar F (i.e. King B media) for enhancing fluorescein production (1). HiFluoro Pseudomonas Agar Base is devised based on the formula described by King et al. (1) except fluorogenic mixture. It is used as the selective medium for the isolation of P. aeruginosa from pus, sputum and drains etc.Cetrimide (Cetyltrimethylammonium bromide) is incorporated in the medium to inhibit bacteria other than P. aeruginosa. It acts as a quaternary ammonium compound, cationic detergent that causes nitrogen and phosphorus to be released from bacterial cells other than P. aeruginosa. P. aeruginosa cleaves the fluorogenic compound to release the fluorogen which produces a visible fluorescence under long wave UV light.
原理與解釋
銅綠假單胞菌（也稱為Pseudomonas pyocyanea）是革蘭氏陰性，需氧，桿狀細菌。與其他假單胞菌一樣，銅綠假單胞菌分泌多種色素，包括綠膿菌素（藍綠色），熒光素（黃綠色和熒光色）和卟啉素（紅棕色）。 King等人開發了用於增強綠膿菌素和嗜熱素產生的假單胞菌瓊脂P（即King A培養基）和用於增強熒光素產生的假單胞菌瓊脂F（即King B培養基）（1）。 基於King等人描述的公式設計了高氟假單胞菌瓊脂基質。 （1）除氟化混合物外。 它被用作從膿液，痰液和排泄物中分離銅綠假單胞菌的選擇性培養基。在培養基中加入吖啶酰亞胺（十六烷基三甲基溴化銨）以抑制銅綠假單胞菌以外的細菌。它作為季銨化合物，陽離子去污劑，使銅和磷從銅綠假單胞菌以外的細菌細胞中釋放出來。銅綠假單胞菌切割熒光化合物以釋放熒光素，其在長波紫外光下產生可見熒光。
銅綠假單胞菌（也稱為Pseudomonas pyocyanea）是革蘭氏陰性，需氧，桿狀細菌。與其他假單胞菌一樣，銅綠假單胞菌分泌多種色素，包括綠膿菌素（藍綠色），熒光素（黃綠色和熒光色）和卟啉素（紅棕色）。 King等人開發了用於增強綠膿菌素和嗜熱素產生的假單胞菌瓊脂P（即King A培養基）和用於增強熒光素產生的假單胞菌瓊脂F（即King B培養基）（1）。 基於King等人描述的公式設計了高氟假單胞菌瓊脂基質。 （1）除氟化混合物外。 它被用作從膿液，痰液和排泄物中分離銅綠假單胞菌的選擇性培養基。在培養基中加入吖啶酰亞胺（十六烷基三甲基溴化銨）以抑制銅綠假單胞菌以外的細菌。它作為季銨化合物，陽離子去污劑，使銅和磷從銅綠假單胞菌以外的細菌細胞中釋放出來。銅綠假單胞菌切割熒光化合物以釋放熒光素，其在長波紫外光下產生可見熒光。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌，
HiMedia 顯色培養基產品 更多元，更豐富，品質優越。