HiMedia – M1353andM1353R HiCrome™ UTI Agar

Principle and Interpretation
Urinary tract infections are bacterial infections affecting parts of urinary tract. The common symptoms of urinary tract infection are urgency and frequency of micturition, with associated discomfort or pain. The common condition is cystitis, due to infection of the bladder with a uropathogenic bacterium, which most frequently is Escherichia coli, but sometimes Staphylococcus saprophyticus or especially in hospital-acquired infections, Klebsiella species, Proteus mirabilis, other coliforms, Pseudomonas aeruginosa or Enterococcus faecalis(1). HiCrome™ UTI Agar is formulated on basis of work carried out by Pezzlo (5) Wilkie et al (7), Friedman et al (2), Murray et al (4), Soriano and Ponte (6) and Merlino et al (3). These media are recommended for the detection of urinary tract pathogens where HiCrome™ UTI Agar has broader application as a general nutrient agar for isolation of various microorganisms. It facilitates and expedites the identification of some gram-negative bacteria and some gram-positive bacteria on the basis of different contrasted colony colours produced by reactions of genus or species specific enzymes with two chromogenic substrates. The chromogenic substrates are specifically cleaved by enzymes produced by Enterococcus species, E. coli and coliforms. Presence of amino acids like phenylalanine and tryptophan from peptones helps for detection of tryptophan deaminase activity, indicating the presence of Proteus species, Morganella species and Providencia species. One of the chromogenic substrate is cleaved by b-glucosidase possessed by enterococci resulting in formation of blue colonies. E. coli produce pink-purple colonies due to the enzyme b-D-galactosidase that cleaves the other chromogenic substrate. Further confirmation of E. coli can be done by performing the indole test. Coliforms produce purple coloured colonies due to cleavage of both the chromogenic substrate. Colonies of Proteus, Morganella and Providencia species appear brown because of tryptophan deaminase activity. Peptone or peptone special provides nitrogenous, carbonaceous compounds long chain amino acids and other essential growth nutrients. This medium can be made selective by supplementation with antibiotics for detecting microorganisms associated with hospital borne infections.

原理與解釋
尿路感染是影響泌尿道部分的細菌感染。尿路感染的常見症狀是排尿的緊迫性和頻率，伴有不適或疼痛。常見病是膀胱炎，由於膀胱感染了尿路感染細菌，最常見的是埃希氏菌屬大腸桿菌，但有時是腐生葡萄球菌或尤其是醫院獲得性感染，克雷伯氏菌，奇異變形桿菌，其他大腸桿菌，銅綠假單胞菌或糞腸球菌（1）。 HiCrome™UTI瓊脂是根據Pezzlo（5）Wilkie等人（7），Friedman等人（2），Murray等人（4），Soriano和Ponte（6）以及Merlino等人（3）開展的工作制定的。 ）。建議使用這些媒體檢測尿路病原體，其中HiCrome™UTI瓊脂作為一般營養瓊脂用於分離各種微生物，具有更廣泛的應用。它基於由屬或物種特異性酶與兩種生色底物的反應產生的不同對比菌落顏色，促進和加速鑑定一些革蘭氏陰性細菌和一些革蘭氏陽性細菌。顯色底物被產生的酶特異性裂解由腸球菌屬，大腸桿菌和大腸桿菌組成。來自蛋白腖的氨基酸如苯丙氨酸和色氨酸的存在有助於檢測色氨酸脫氨酶活性，表明存在變形桿菌屬物種，摩根氏菌屬物種和普羅威登斯菌種。 其中一個生色底物被腸球菌所具有的β-葡糖苷酶切割，導致藍色菌落的形成。由於b-D-半乳糖苷酶切割其他生色底物，大腸桿菌產生粉紅色 - 紫色菌落。可以通過進行吲哚試驗來進一步確認大腸桿菌。由於顯色的裂解，大腸桿菌產生紫色的菌落基質。由於色氨酸脫氨酶活性，Proteus，Morganella和Providencia物種的菌落呈棕色。蛋白腖或蛋白腖特別提供含氮，含碳化合物長鏈氨基酸和其他必需的生長營養素。 通過補充抗生素可以使該培養基具有選擇性，用於檢測與醫院感染相關的微生物。