HIMEDIA HiCrome 顯色培養基（／產色培養基／呈色培養基） |
顯色培養基（／產色培養基／呈色培養基）
( Chromogenic / Fluorogenic Culture Media )
顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
(一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形，
藉此直接觀察菌落顏色即可對菌種做出鑑定，
其反應的靈敏度和特異性大大優於傳統培養基。
顯色培養基通常為乾粉狀，容易儲存。
加蒸餾水溶解後，部分產品無須高壓滅菌，培養時間依具體培養基而定，
通常是18-24小時，比傳統時間顯著縮短。
HiMedia – M1297AR HiCrome™ Candida Differential Agar Base |
Recommended as a selective and differential medium for rapid isolation and identification of Candida species from mixed cultures.
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Principle and Interpretation
Perry and Miller (1) reported that Candida albicans produces an enzyme b -N-acetyl- galactosaminidase and according to Rousselle et al (2) incorporation of chromogenic or fluorogenic hexosaminidase substrates into the growth medium helps in identification of C. albicans isolates directly on primary isolation. HiCrome™ Candida Differential Agar Base incorporates two chromogenes X-NAG which detects the activity of hexosaminidase and BCIP which detects phosphatase activity. HiCrome™ Candida Differential Agar Base is a selective and differential medium, which facilitates rapid isolation of yeasts from mixed cultures and allows differentiation of Candida species namely C.albicans, C.krusei, C.tropicalis and C.glabrata on the basis of colouration and colony morphology. On this medium results are obtained within 48 hours and it is useful for the rapid and presumptive identification of common yeasts in Mycology and Clinical Microbiology Laboratory. Peptone provides nitrogenous, carbonaceous compounds and other essential growth nutrients. Chloramphenicol from the supplement suppresses the accompanying bacterial flora. C.albicans appear as light green coloured smooth colonies, C.tropicalis appear as blue to metallic blue coloured raised colonies. C.glabrata, C.kefyr, C.parapsilosis colonies appear as cream to white, beige/yellow due to natural pigmentation and some alkaline phosphatase activity, while C.krusei appear as pink-purple, fuzzy, dry colonies.
Perry and Miller (1) reported that Candida albicans produces an enzyme b -N-acetyl- galactosaminidase and according to Rousselle et al (2) incorporation of chromogenic or fluorogenic hexosaminidase substrates into the growth medium helps in identification of C. albicans isolates directly on primary isolation. HiCrome™ Candida Differential Agar Base incorporates two chromogenes X-NAG which detects the activity of hexosaminidase and BCIP which detects phosphatase activity. HiCrome™ Candida Differential Agar Base is a selective and differential medium, which facilitates rapid isolation of yeasts from mixed cultures and allows differentiation of Candida species namely C.albicans, C.krusei, C.tropicalis and C.glabrata on the basis of colouration and colony morphology. On this medium results are obtained within 48 hours and it is useful for the rapid and presumptive identification of common yeasts in Mycology and Clinical Microbiology Laboratory. Peptone provides nitrogenous, carbonaceous compounds and other essential growth nutrients. Chloramphenicol from the supplement suppresses the accompanying bacterial flora. C.albicans appear as light green coloured smooth colonies, C.tropicalis appear as blue to metallic blue coloured raised colonies. C.glabrata, C.kefyr, C.parapsilosis colonies appear as cream to white, beige/yellow due to natural pigmentation and some alkaline phosphatase activity, while C.krusei appear as pink-purple, fuzzy, dry colonies.
原理與解釋
Perry和Miller（1）報導白色念珠菌產生酶b-N-乙酰基 - 半乳糖胺酶，並且根據Rousselle等人的研究（2）將生色或熒光己糖胺酶底物摻入生長培養基中有助於直接鑑定白色念珠菌分離株。初級隔離。 HiCrome™念珠菌差異瓊脂基質含有兩種髮色團X-NAG，可檢測氨基己糖苷酶和檢測磷酸酶活性的BCIP的活性。 HiCrome™念珠菌差異瓊脂基質是一種選擇性和差異性培養基，有助於從混合培養物中快速分離酵母，並可根據著色和分離念珠菌屬物種C.albicans，C.krusei，C.tropicalis和C.glabrata。菌落形態。在該培養基上，在48小時內獲得了結果，並且它可用於在真菌學和臨床微生物學實驗室中快速和假定地鑑定常見酵母。 蛋白腖提供含氮，含碳化合物和其他必需的生長營養素。來自補充劑的氯黴素抑制伴隨的細菌菌群。 C.albicans顯示為淺綠色的光滑菌落，C.tropicalis顯示為藍色至金屬藍色的凸起菌落。 C.glabrata，C.kefyr，C.parapsilosis菌落由於天然色素沉著和一些鹼性磷酸酶活性而呈乳白色，米色/黃色，而C.krusei則呈現粉紅色 - 紫色，模糊，乾燥的菌落。
Perry和Miller（1）報導白色念珠菌產生酶b-N-乙酰基 - 半乳糖胺酶，並且根據Rousselle等人的研究（2）將生色或熒光己糖胺酶底物摻入生長培養基中有助於直接鑑定白色念珠菌分離株。初級隔離。 HiCrome™念珠菌差異瓊脂基質含有兩種髮色團X-NAG，可檢測氨基己糖苷酶和檢測磷酸酶活性的BCIP的活性。 HiCrome™念珠菌差異瓊脂基質是一種選擇性和差異性培養基，有助於從混合培養物中快速分離酵母，並可根據著色和分離念珠菌屬物種C.albicans，C.krusei，C.tropicalis和C.glabrata。菌落形態。在該培養基上，在48小時內獲得了結果，並且它可用於在真菌學和臨床微生物學實驗室中快速和假定地鑑定常見酵母。 蛋白腖提供含氮，含碳化合物和其他必需的生長營養素。來自補充劑的氯黴素抑制伴隨的細菌菌群。 C.albicans顯示為淺綠色的光滑菌落，C.tropicalis顯示為藍色至金屬藍色的凸起菌落。 C.glabrata，C.kefyr，C.parapsilosis菌落由於天然色素沉著和一些鹼性磷酸酶活性而呈乳白色，米色/黃色，而C.krusei則呈現粉紅色 - 紫色，模糊，乾燥的菌落。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌，
HiMedia 顯色培養基產品 更多元，更豐富，品質優越。