HIMEDIA HiCrome 顯色培養基（／產色培養基／呈色培養基） |
顯色培養基（／產色培養基／呈色培養基）
( Chromogenic / Fluorogenic Culture Media )
顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
(一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形，
藉此直接觀察菌落顏色即可對菌種做出鑑定，
其反應的靈敏度和特異性大大優於傳統培養基。
顯色培養基通常為乾粉狀，容易儲存。
加蒸餾水溶解後，部分產品無須高壓滅菌，培養時間依具體培養基而定，
通常是18-24小時，比傳統時間顯著縮短。
HiMedia – M1296andM1466 HiCrome™ Salmonella Agar /HiCrome™ Improved Salmonella Agar |
Recommended for the simultaneous detection of Salmonella and Escherichia coli from food, water and clinical samples.
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Principle and Interpretation
Salmonella species have been isolated from humans and almost all animals throughout the world. They cause many types of infections from mild, self-limiting gastroenteritis to life threatening typhoid fever. Salmonella Typhi and Salmonella Paratyphi A & B cause gastroenteritis, bacteremia and enteric fever, Salmonella Choleraesuis causes gastroenteritis and enteric fever, especially in children. Salmonella Typhimurium is the most frequently isolated serotype of Salmonella (2). HiCrome™ Salmonella Agar medium is a modification of the original formulation of Rambach (3) and is used for the differentiation of Salmonella species from other enteric bacteria. Rambach formulation differentiates Salmonella based on propylene glycol utilization and presence of a chromogenic indicator. However, HiCrome™ Salmonella Agar medium uses only a chromogenic mixture for identification and differentiation of Salmonella species. Peptone, peptone special and yeast extract provides nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other essential growth nutrients. Escherichia coli and Salmonella are easily distinguishable due to their colony characteristics. Salmonella forms light purple coloured colonies with a purple halo on (M1296) and pink to red colonies on (M1466). E. coli exhibits a characteristic blue colour, due to presence of the enzyme b-glucuronidase. Other organisms form colourless colonies. The characteristic light purple and blue colour is due to the chromogenic mixture (1). Bile salts mixture or sodium deoxycholate inhibits grampositive organisms.
Salmonella species have been isolated from humans and almost all animals throughout the world. They cause many types of infections from mild, self-limiting gastroenteritis to life threatening typhoid fever. Salmonella Typhi and Salmonella Paratyphi A & B cause gastroenteritis, bacteremia and enteric fever, Salmonella Choleraesuis causes gastroenteritis and enteric fever, especially in children. Salmonella Typhimurium is the most frequently isolated serotype of Salmonella (2). HiCrome™ Salmonella Agar medium is a modification of the original formulation of Rambach (3) and is used for the differentiation of Salmonella species from other enteric bacteria. Rambach formulation differentiates Salmonella based on propylene glycol utilization and presence of a chromogenic indicator. However, HiCrome™ Salmonella Agar medium uses only a chromogenic mixture for identification and differentiation of Salmonella species. Peptone, peptone special and yeast extract provides nitrogenous and carbonaceous compounds, long chain amino acids, vitamins and other essential growth nutrients. Escherichia coli and Salmonella are easily distinguishable due to their colony characteristics. Salmonella forms light purple coloured colonies with a purple halo on (M1296) and pink to red colonies on (M1466). E. coli exhibits a characteristic blue colour, due to presence of the enzyme b-glucuronidase. Other organisms form colourless colonies. The characteristic light purple and blue colour is due to the chromogenic mixture (1). Bile salts mixture or sodium deoxycholate inhibits grampositive organisms.
原理與解釋
沙門氏菌已從人類和世界各地的幾乎所有動物中分離出來。它們引起多種類型的感染，從輕度，自限性胃腸炎到危及生命的傷寒。 傷寒沙門氏菌和副傷寒沙門氏菌A和B引起腸胃炎，菌血症和腸炎，霍亂沙門氏菌引起胃腸炎和腸熱，尤其是兒童。鼠傷寒沙門氏菌是最常見的沙門氏菌血清型（2）。 HiCrome™沙門氏菌瓊脂培養基是Rambach（3）原始配方的改良劑，用於區分沙門氏菌和其他腸道細菌。 Rambach配方基於丙二醇利用和存在顯色指示劑來區分沙門氏菌。然而，HiCrome™沙門氏菌瓊脂培養基僅使用生色混合物進行鑑定和鑑定 沙門氏菌的分化。 蛋白腖，蛋白腖專用和酵母提取物提供含氮和含碳化合物，長鏈氨基酸，維生素和其他必需的生長營養素。 由於其菌落特徵，大腸桿菌和沙門氏菌很容易區分。沙門氏菌形成淺紫色的菌落，在（M1296）上有紫色光環，在（M1466）上有粉紅色到紅色的菌落。 由於存在酶β-葡醣醛酸糖苷酶，大腸桿菌顯示出特徵性的藍色。其他生物形成無色菌落。特徵淺紫色和藍色是由於髮色混合物（1）。膽汁鹽混合物或脫氧膽酸鈉抑制革蘭氏陽性菌。
沙門氏菌已從人類和世界各地的幾乎所有動物中分離出來。它們引起多種類型的感染，從輕度，自限性胃腸炎到危及生命的傷寒。 傷寒沙門氏菌和副傷寒沙門氏菌A和B引起腸胃炎，菌血症和腸炎，霍亂沙門氏菌引起胃腸炎和腸熱，尤其是兒童。鼠傷寒沙門氏菌是最常見的沙門氏菌血清型（2）。 HiCrome™沙門氏菌瓊脂培養基是Rambach（3）原始配方的改良劑，用於區分沙門氏菌和其他腸道細菌。 Rambach配方基於丙二醇利用和存在顯色指示劑來區分沙門氏菌。然而，HiCrome™沙門氏菌瓊脂培養基僅使用生色混合物進行鑑定和鑑定 沙門氏菌的分化。 蛋白腖，蛋白腖專用和酵母提取物提供含氮和含碳化合物，長鏈氨基酸，維生素和其他必需的生長營養素。 由於其菌落特徵，大腸桿菌和沙門氏菌很容易區分。沙門氏菌形成淺紫色的菌落，在（M1296）上有紫色光環，在（M1466）上有粉紅色到紅色的菌落。 由於存在酶β-葡醣醛酸糖苷酶，大腸桿菌顯示出特徵性的藍色。其他生物形成無色菌落。特徵淺紫色和藍色是由於髮色混合物（1）。膽汁鹽混合物或脫氧膽酸鈉抑制革蘭氏陽性菌。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌，
HiMedia 顯色培養基產品 更多元，更豐富，品質優越。