HiMedia – M2011 HiCrome™ Rapid ECC Broth

Principle and Interpretation
HiCrome™ Rapid ECC Broth is designed for detection and confirmation of Escherichia coli and other coliforms from water samples. The major microbial water contaminants are coliforms include Escherichia coli, Klebsiella pneumoniae, Salmonella, Citrobacter, Vibrio, and Pseudomonas (1). This test was designed for the rapid detection and differentiation of these organisms. Peptone special provides nitrogen and carbon source, long chain amino acids, vitamins and other essential growth nutrients. Phosphates buffer the medium. Lactose is the fermentable carbohydrate and phenol red is the indicator. Lactose fermenting organisms gives yellow colour to the medium while lactose non-fermentors gives pink to red colour. The chromogenic substrate is used to detect the presence of ß-D-glucuronidase produced by E.coli thus imparting blue colour to the medium. However since E.coli also ferments lactose, the presence of E.coli is indicated by bluish gren to green colour. The detection of H2S production is enhanced by the presence of specific H2S detectors. The medium turns black in case of H2S producers such as Salmonella, Citrobacter etc are present. The phosphate salts provide buffering action for rapid growth of coliforms. Sodium chloride helps to maintain the osmotic balance. Selective mix present in the medium suppresses the growth of gram positive microorganisms. Recovery of these pathogens is faster and reliable.

原理與解釋
HiCrome™Rapid ECC Broth設計用於檢測和確認水樣中的大腸桿菌和其他大腸桿菌。主要的微生物水污染物是大腸桿菌，包括大腸桿菌，肺炎克雷伯菌，沙門氏菌，檸檬酸桿菌，弧菌和假單胞菌（1）。該測試旨在快速檢測和區分這些生物。 蛋白腖特別提供氮和碳源，長鏈氨基酸，維生素和其他必需的生長營養素。磷酸鹽緩衝培養基。乳糖是可發酵的碳水化合物，酚紅是指示劑。乳糖發酵生物為培養基提供黃色，而乳糖非發酵生物呈現粉紅色至紅色。顯色底物用於檢測由大腸桿菌產生的β-D-葡醣醛酸糖苷酶的存在，從而賦予培養基藍色。然而，由於大腸桿菌也發酵乳糖，大腸桿菌的存在表現為藍色的綠色到綠色。特定H2S檢測器的存在增強了H2S產生的檢測。 如果存在沙門氏菌，檸檬酸桿菌等H2S生產者，則培養基變黑。磷酸鹽為大腸桿菌的快速生長提供緩衝作用。氯化鈉有助於維持滲透平衡。培養基中存在的選擇性混合物抑制革蘭氏陽性微生物的生長。這些病原體的恢復更快更可靠。