HIMEDIA HiCrome 顯色培養基(/產色培養基/呈色培養基) |
顯色培養基(/產色培養基/呈色培養基)
                                
( Chromogenic / Fluorogenic Culture Media )
顯色培養基一般是在培養基中加入檢測特定菌種的特殊性底物。
        (一種或多種顯色劑(目測) 或 熒光顯色劑(紫外燈照射觀察) )
該特殊性底物 與 特定微生物自身代謝產生的酶 會產生顯色(產色)情形,
藉此直接觀察菌落顏色即可對菌種做出鑑定,
其反應的靈敏度和特異性大大優於傳統培養基。
顯色培養基通常為乾粉狀,容易儲存。
加蒸餾水溶解後,部分產品無須高壓滅菌,培養時間依具體培養基而定,
通常是18-24小時,比傳統時間顯著縮短。
HiMedia – M1951_HiCrome™ M-Coliform Differential Agar Base |
Recommended as a selective and differential agar for the detection of coliform bacteria using membrane filtration technique.
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Principle and Interpretation
HiCrome™ M-Coliform Differential Agar Base is based on coliform enumeration medium, M-FC Agar (1). This medium was modified for detection and enumeration of total coliforms by addition of Monensin supplement to improve the recovery of injured coliforms (2). Peptone, Tryptone and Yeast extract provides nitrogeneous compounds, carbonaceous compounds, long chain amino acids and other growth nutrients and vitamins. Lactose is the fermentable carbohydrate. Monensin and sodium deoxycholate acts as selective agents, inhibiting Gram-positive bacteria. Aniline blue forms the indicator system of the medium. The chromogenic substrate is utilized by E.coli which detects the presence of b-glucuronidase. The medium helps injured coliforms to grow in the presence of selective agents.
HiCrome™ M-Coliform Differential Agar Base is based on coliform enumeration medium, M-FC Agar (1). This medium was modified for detection and enumeration of total coliforms by addition of Monensin supplement to improve the recovery of injured coliforms (2). Peptone, Tryptone and Yeast extract provides nitrogeneous compounds, carbonaceous compounds, long chain amino acids and other growth nutrients and vitamins. Lactose is the fermentable carbohydrate. Monensin and sodium deoxycholate acts as selective agents, inhibiting Gram-positive bacteria. Aniline blue forms the indicator system of the medium. The chromogenic substrate is utilized by E.coli which detects the presence of b-glucuronidase. The medium helps injured coliforms to grow in the presence of selective agents.
原理與解釋
HiCrome™M-Coliform差異瓊脂基質基於大腸菌群計數培養基,M-FC瓊脂(1)。 修改該培養基用於通過添加莫能菌素補充劑來檢測和計數總大腸菌群,以改善受損大腸菌群的恢復(2)。 蛋白腖,胰蛋白腖和酵母提取物提供硝基化合物, 含碳化合物,長鏈氨基酸和其他生長營養素和維生素。 乳糖是可發酵的碳水化合物。 莫能菌素和脫氧膽酸鈉作為選擇劑起作用,抑制革蘭氏陽性細菌。 苯胺藍形成了培養基的指示系統。 大腸桿菌利用顯色底物檢測β-葡醣醛酸糖苷酶的存在。 培養基可幫助受傷的大腸菌在選擇性藥劑存在下生長。
HiCrome™M-Coliform差異瓊脂基質基於大腸菌群計數培養基,M-FC瓊脂(1)。 修改該培養基用於通過添加莫能菌素補充劑來檢測和計數總大腸菌群,以改善受損大腸菌群的恢復(2)。 蛋白腖,胰蛋白腖和酵母提取物提供硝基化合物, 含碳化合物,長鏈氨基酸和其他生長營養素和維生素。 乳糖是可發酵的碳水化合物。 莫能菌素和脫氧膽酸鈉作為選擇劑起作用,抑制革蘭氏陽性細菌。 苯胺藍形成了培養基的指示系統。 大腸桿菌利用顯色底物檢測β-葡醣醛酸糖苷酶的存在。 培養基可幫助受傷的大腸菌在選擇性藥劑存在下生長。
HiMedia Laboratories 的顯色培養基 產品以HiCrome為開頭命名
      
相較於 法國Chromagar、德國Merck、英國Oxoid、美國Remel 其他顯色培養基品牌,
      
HiMedia 顯色培養基產品 更多元,更豐富,品質優越。